Pokémon gender was a concept introduced in Pokémon Vortex Version 3. Almost all species of Pokémon were assigned a gender, male or female, but Legendary Pokemon do not have any gender. Gender makes no difference in the stats of a Pokémon, but the gender could affect evolution paths. One such example is 
Nidoran (M) and 
Nidoran (F).
Gender-based evolutions
Certain Pokemon have to be a certain gender to evolve. Below is a list of gender and items needed for evolution.
| Base Form | Item Required | Evolved Form |
 Kirlia |
 &  Male & Dawn Stone |
 Gallade |
 Snorunt |
 & Female & Dawn Stone |
 Froslass |
 Burmy (Plant) |
&  Female & Level 20 |
 Wormadam (Plant) |
 Burmy (Sand) |
& Female & Level 20 |
 Wormadam (Sand) |
 Burmy (Steel) |
& Female & Level 20 |
 Wormadam (Steel) |
Burmy (Plant) |
& Male & Level 20 |
 Mothim |
Burmy (Sand) |
& Male & Level 20 |
Mothim |
Burmy (Steel) |
& Male & Level 20 |
Mothim |
 Combee |
& Female & Level 21 |
 Vespiquen |
 Espurr |
& Female & Level 25 |
 Meowstic (F) |
Espurr |
& Male & Level 25 |
 Meowstic (M) |
 Salandit |
& Female & Level 33 |
 Salazzle |