Pokémon gender was a concept introduced in Pokémon Vortex Version 3. Almost all species of Pokémon were assigned a gender, male or female, but Legendary Pokemon do not have any gender. Gender makes no difference in the stats of a Pokémon, but the gender could affect evolution paths. One such example is
Nidoran (M) and
Nidoran (F).
Single-gender Pokémon
Gender-based evolutions
Certain Pokemon have to be a certain gender to evolve. Below is a list of gender and items needed for evolution.
| Base Form | Item Required | Evolved Form |
| Burmy (Plant) Burmy (Plant) |
& Female & Level 20 |
Wormadam (Plant) Wormadam (Plant) |
| Burmy (Sand) Burmy (Sand) |
& Female & Level 20 |
Wormadam (Sand) Wormadam (Sand) |
| Burmy (Steel) Burmy (Steel) |
& Female & Level 20 |
Wormadam (Steel) Wormadam (Steel) |
| Burmy (Plant) Burmy (Plant) |
& Male & Level 20 |
Mothim Mothim |
| Burmy (Sand) Burmy (Sand) |
& Male & Level 20 |
Mothim Mothim |
| Burmy (Steel) Burmy (Steel) |
& Male & Level 20 |
Mothim Mothim |
| Combee Combee |
& Female & Level 21 |
Vespiquen Vespiquen |
| Kirlia Kirlia |
& Male & Dawn Stone |
Gallade Gallade |
| Nidoran (F) Nidoran (F) |
& Female & Level 16 |
Nidorina Nidorina |
| Nidoran (M) Nidoran (M) |
& Male & Level 16 |
Nidorino Nidorino |
| Snorunt Snorunt |
& Female & Dawn Stone |
Froslass Froslass |